1. Exercise 46: MPN
    1. Describe the principle and practice of a MPN test
    2. What organism is being identified in water samples, and why is this organism used?
    3. What is the presumptive test?
    4. What is the confirmed test?
    5. Interpret the results of MPN tests of water samples
2. Exercise 42: Disinfectants
    1. Distinguish between bacteriostatic and bacteriocidal, and identify an experimental method that distinguishes the two
    2. Identify physical factors that influence the effectiveness of disinfectants
    3. Interpret data from a study of the effectiveness of disinfectants
3. Exercise 43: Antibiotics
    1. Measure the zone of inhibition, and use a table to determine if the organism is resistant, intermediate, or sensitive to the antibiotic
    2. List the factors that must be controlled in the Kirby-Bauer test to ensure accuracy
    3. Discuss factors that can increase the resistance of a microbe to an antibiotic
4. Exercise 53: Body sample
    1. Interpret results of the following tests: hemolysis on blood agar, catalase, Gram stain, coagulase, TSI, SIM, citrate and MRVP
    2. Use the results of tests and a flow chart or table to identify common bacteria of the throat, skin and rectum
5. Exercise 50: Food Sample
    1. Identify methods for the proper handling and storage of food
    2. Interpret the results of a heterotrophic plate count and determine if the food is contaminated, or if the bacteria present are normal
    3. Determine the number of bacteria per ml of a food sample
6. Exercise 51: Milk Sample
    1. Differentiate between pasteurization and sterilization
    2. Explain why milk is typically pasteurized instead of sterilized
    3. Describe two methods of pasteurization
    4. What is the source of coliforms found in milk?
    5. Determine the bacteria per ml of milk
7. Safety/waste disposal
    1. Describe the specific disposal techniques for plates, tubes, pipets, slides and paper waste
    2. Describe proper laboratory safety procedures
8. Flowcharting
    1. Given data in a table, construct a flow chart to separate all organisms
    2. Given a flow chart and results of biochemical tests and stains, identify an unknown organism
9. Unknowns
    1. Describe the procedures and interpret the results of any of the tests used in identifying the unknowns:
        1. MRVP
        2. Carbohydrate fermentation
        3. TSI
        4. SIM
        5. Citrate
        6. Urea
        7. Catalase
        8. Oxidase
        9. Coagulase
        10. SIM
        11. Gelatin
        12. Hemolysis on blood agar
    2. Indicate the reason for confirming growth before interpreting the results of a biochemical test
    3. State the reason for using isolated colonies in setting up the biochemical tests
10. Pronunciation
    1. Be able to correctly pronounce all of the organisms used in the unknowns

For questions, comments and additional information, contact mfhicks@pstcc.edu
Last Updated: June 2, 2003
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