Biology 2130 Microbiology

Class Hours: 3                                                                              Credit Hours: 4


Laboratory Hours: 4                                                                     Date Revised: Spring 2015


Catalog Course Description:

Microbiology:  An introductory course in microbiology dealing with bacteria, fungi, yeast and viruses to include discussions of cell structure, identification, taxonomy, metabolism, genetics, resistance, infection, and disease.  Course includes three hours of lecture and four hours of laboratory applications each week.


Entry Level Standards:

High school biology; students are expected to read and write at the college level.



CHM 1010 or 1110; or BIO 2010; or BIO 1110



Bauman, Robert W., Microbiology With Diseases by Body System, Fourth Edition, Pearson-Benjamin Cummings. (Hard copy or electronic version is acceptable)


Leboffe, Michael J. and Pierce, Burton E.  Microbiology Laboratory Theory and Application.,2nd edition, Customized for PSCC, Morton.


Lab Manual must be new.  Data sheets from used lab manuals will NOT be given grades.


I.          Week/Unit/Topic Basis:



Lecture Chapter(s):


Lab Exercises


M, 1/19 OUT

Jan 20 - 23


A Brief History of Microbiology

1st Lab Day:  2-1,2-2

2nd Lab Day:  3-1


Jan 26-  30



Cell Structure and Function

1st Lab Day:   3-3

2nd Lab Day:  3-4


Feb 2 - 6


Staining and Classification

1st Lab Day:  3-6

2nd Lab Day:  3-9


Feb 9 - 13

Exam I (1, 3, 4) 6

Microbial Nutrition and Growth

1st Lab Day:  3-12

2nd Lab Day 2-6, 2-7, 2-8


Feb 16 - 20

6 cont.


Controlling Microbial Growth in the Environment

1st Lab Day:  6-2

2nd Lab Day:   7-6


Feb 23 - 27

9 cont.


Controlling Microbial Growth in the Body: Antimicrobial Drugs

1st Lab Day: 2-13 

2nd Lab Day: 7-2


Mar 2 - Mar 6

10 cont.

Exam II (6, 9, 10)


1st Lab Day: Review

2nd Lab Day: Lab Practical I


Mar 9 - Mar 13





Mar 16  -20


Microbial Metabolism

1st Lab Day: 1-5, 4-1, 4-5

2nd Lab Day: 5-1, 5-2, 5-3


Mar 23 – Mar 27


5 cont.


Microbial Genetics

1st Lab Day: 5-8, 5-9

2nd Lab Day: 5-18


Mar 30 – Apr 2

F, April 3 OUT

7 cont.


1st Lab Day: 5-4, 5-5, 5-6

2nd Lab Day: OUT


Apr 6 - 10

Exam III (5, 7)


Recombinant DNA Technology

1st Lab Day: 5-21, 5-23

2nd Lab Day  Review


Apr 13 - 17

8 cont.


Characterizing and Classifying Viruses

1st Lab Day Lab Practical II

2nd Lab Day:  Unknown


Apr 20 – Apr 24

13 cont.


Infection, Infectious Diseases and


1st Lab Day:  Unknown

2nd Lab Day:  Unknown


Apr 27 –  May 1

14 cont.

Exam IV (8,13,14)


1st Lab Day:  Unknown

2nd Lab Day: Unknown


May 4 - 7

Comprehensive Final




II.  Course Goals*:


The course will

A.            Provide a working knowledge of basic staining and culturing techniques and concepts. V.1, V.2

B.            Expand the student's familiarity with prokaryotic and eukaryotic characteristics.  V.3, V.4

C.            Enhance understanding of microbial metabolism and growth. V.3, V.4

D.            Provide a means for learning to classify microorganisms. V.3, V.4

E.            Expand student understanding of epidemiology, pathogenicity and drug treatment of specified microbial organisms. V.4

F.            Provide an opportunity for group interaction and problem solving.

G.            Expand their knowledge of the principles of immunology and applied microbiology.   V.3, V.5, V4

H.            Enhance the students ability to read and apply critical thinking to topics in the field of microbiology V.1, V.5

I.              Guide them in becoming familiar with resources available on recent research and current information in the library in the field of microbiology. VII.3, VII.4, VII.5

J.             Provide students with the opportunity to collect and utilize data in graphs and reports. VII.2

K.            Provide opportunities to become comfortable with communication in the field of microbiology and particular the areas that apply to Allied Health. VII.1


* Roman numerals after Coarse Goals refer to goals of the Natural and Behavioral Sciences Department.


III.        Expected Student Learning Outcomes*:


Students will be able to:

1.             Locate and evaluate related scientific information in the ERC and on the World Wide Web.  I

2.             Use related equipment and tools for making biological measurements and observations.   A

3.             Use internet course list serve to share information pertaining to the course with classmates.   H

4.             Collect data, generate graphs and tables of the collected data, summarize the data, draw conclusions from the data, and apply these conclusions to related situations.   J

5.             Read and critique scientific writings.   I

6.             Develop a vocabulary that allows them to communicate more effectively with their health care providers as well as in preparing for health care professions.  K

7.             Participate in laboratory exercises which develop teamwork, problem solving skills and data analysis.  F, J

8.             Utilize skills and procedures developed in the laboratory to design and implement a plan to identify unknown microorganisms.  D

9.         Identify microbes through the use of cultures and staining techniques. A

10.        Describe procaryotic cell characteristics as they relate to organism identification. B

11.        Understand microbe metabolism and growth and their controlling factors. C

12.        Describe genetic operations within microbes and application of these operations as they apply to technology. H

13.        Understand the mechanisms of classifying microbes and viruses, bacteria, protists, fungi, and helminths. D

14.        Recognize the ramifications of drug action and treatment for specified microbes. E


* Capital letters listed after Expected Student Learning Outcomes reference course goals listed above.


IV.        Evaluation:


A. Lecture Expectations:


Testing Procedures: 51% (500 points)

Lecture assignments consist of 4 exams worth 75 pts. each (300 pts total); a comprehensive final exam  worth 100 pts;  5 abstracts worth 10 pts. each (50 pts.); and other assignments as indicated by the instructor (50 pts).           Assignments completed in a previous semester will not be accepted.


Exams: The four exams will be non-cumulative, although students are expected to retain basic information acquired in previous chapters.  Exams will consist of a possible combination of multiple choice, matching, short answer, true or false and essay questions.    Make-up exams are at the discretion of the lecture instructor.  The final exam will be conducted in the classroom on the assigned final exam day.  The final exam is not optional! 


Abstracts:  Students will write abstracts of current articles pertaining to microbiological issues covered in the course, following guidelines discussed in class.  Due dates will be announced by the lecture instructor.


Late Assignments:  Assignments must be turned in before class starts on the day they are due.  Any assignment turned in late will have 20% deducted from the overall grade.  Assignments will not be accepted after the end of the day (11:55 p.m.) they are due without permission from the instructor.


B.    Laboratory Expectations:


Testing procedures: 49% (485 points)


Participation in laboratory exercises is mandatory.  Unless otherwise indicated, all laboratory work is to be done individually.  Laboratory assignments points are broken down as follows: laboratory exercises, handouts, quizzes and any other assignments made by the instructor (310 points), practical exams (100 points), and unknown identification (55 points).  The remaining 20 points will be based on attitude, teamwork, technique, lab safety, effort, following directions and showing up on time.


Students must come to the laboratory prepared.  Preparation includes, but is not limited to: 1) reading exercises in advance, 2) turning in completed assignments 3) bringing lab manual, prep sheets and Sharpie to class, 4) and wearing lab coat and safety glasses.  Students MUST wear a full length lab coat in lab at all times!!  Lab coats must be in a zipped lock plastic bag when brought to class and taken out of class in a zip lock plastic bag.   Students supply their own laboratory coat, safety glasses, rubber gloves if desired and permanent sharpie.  Short lab jackets and short sleeved lab coats are NOT acceptableNo food, drink, shorts or open-toed/open-heeled shoes are allowed in lab.   No one will be allowed in lab who is not appropriately dressed and/or does not have goggles and a lab coat in a plastic zip lock bag. 


Laboratory exercises: Exercises will be graded on completeness (including following directions), neatness, and accuracy.  Students will receive no credit for a lab (or the portion of a lab) they miss.  See instructor’s handouts for additional information.


Practical Exams:  Laboratory practical exams will consist of a set of stations.  Stations may contain slides, culture plates, equipment or data from previous labs.  Each station will have one or more questions based on the materials present.  Sample stations and review sessions will be provided in lab before each practical. 


Unknown Identification:  Students will receive a broth containing two organisms which must be isolated and identified.  Students will design and utilize a flow chart of stains and biochemical tests to identify these organisms. The flow chart used during identification, a daily log, as well as a typed exercise report will be submitted for this assignment. 


Missed labs:  Due to the nature of the microbiology labs, missed labs cannot be made up.  If a student misses one day out of the two days an exercise is being worked on in lab, the students may use a lab partner's data or set-up in order to do an Exercise Assignment/Report but the student will only receive a maximum of half credit for the data sheets.  Arrangements can be made to complete the laboratory during another laboratory section in doing the same lab.  There are ABSOLUTELY NO make-ups of laboratory practical exams after the end of the Lab Practical period!


Late Assignments:  Assignments must be turned in before class starts on the day they are due.  Any assignment turned in late will have 20% deducted from the overall grade.  Assignments will not be accepted after the end of the day (11:55 p.m.) they are due without permission from the instructor. Assignments completed in a previous semester will not be accepted.



C.    Grading Scale


The final grade will be based on accumulation of points from both lecture and lab (985 points). 

            883-985 points              90% - 100%                  A

            852-882 points              87% - 89%                    B+

            783-851 points              80% - 86%                    B

            753-782 points              77% - 79%                    C+

            685-752 points              70% - 76%                    C

            586-684 points              60% - 69%                    D

            585 and below               59% and below                         F



V.            Policies:


Attendance:  PSTCC expects students to attend all scheduled instructional activities and to be on time.  The teacher should be notified if the student knows she/he is going to be late or absent. Points will be taken off for coming in late.  Students in all courses must be present for at least 75 percent of their scheduled class and laboratory meetings in order to receive credit for the course.  If a student is absent from class or lab, it is the student's responsibility to make up the missed material.


Maintaining continuous attendance in your classes is very important.  If you are considering dropping or withdrawing from a course, please check with the Financial Aid Office before doing so.  Dropping or withdrawing from a class can adversely affect your financial aid and/or lottery eligibility.

Academic Honesty: Academic misconduct committed either directly or indirectly by an individual or group is subject to disciplinary action. Prohibited activities include but are not limited to the following practices:

Upon discovery of a student's participation in academic misconduct, the instructor will meet with the offending student with evidence of the misconduct. In addition to other possible disciplinary sanctions that may be imposed, the instructor has the authority to assign either (1) an F or zero for the assignment or (2) an F for the course.

Pellissippi State students accept full responsibility for the quality and authenticity of submitted course work. When confronted with evidence of academic misconduct, students may admit their participation and accept the penalty imposed by the instructor. The instructor will inform the department head of the violation, and the department head will forward written notice of the violation to the dean of Student Affairs, who will keep records of the incident.

If the student believes that he/she has been erroneously accused of academic misconduct and if his/her final grade has been lowered as a result, the student may appeal the case through the following procedures:

Accommodations:  If you need accommodations because of a disability, have emergency medical information to share, or need special arrangements in case the building must be evacuated, please inform the instructor immediately.  Students must present current accommodation plan from staff member in Services for Students with Disabilities (SSWD) in order to receive accommodations. Services for Students with Disabilities may be contacted by going to Goins 125, 127 or 131, or Alexander 105 or by phone: 694-6751(Voice/TDY), 539-7153, 539-7091 or 539-7249.


Cell Phone Usage: Use of cell phones in the microbiology classroom is not permitted.  Phones are to be turned off and stored.  In emergency situations, you may speak with the instructor prior to the beginning of a class session about possibly receiving a phone message during class


Class Room Behavior:  The Lecture/Lab Instructor will provide additional information on "do's and don'ts" in classrooms and labs including use of computers during class.


School Closures: In the event of unplanned school closure check the College's web-site for details for closures and/or delays. Students will be responsible for any assignments due or assigned during a closure.


 If classes are canceled by the school for periods of more than a week the student is responsible for going to the class D2L website for information on what assignments to complete during this period.  If D2L is unavailable check the instructor's telephone voice mail for instructions. 


When classes resume, Labs will start with the exercises that were scheduled for the first lab missed.   The exercise schedule will be modified if more than two lab periods are cancelled.







This page is to be signed and turned in to Lecture Instructor on the second day of class.









I have read and understand the syllabus and course expectations for Biology 2130, including the policies regarding missed exams, late assignments, cell phone usage, classroom behavior and academic honesty.